P26: Practical therapeutic applications of ferromagnetic carbon nanotubes in colon cancer treatment

Ireneusz Majsterek1, Dariusz Koza2, Łukasz Szymański2, Grzegorz Raniszewski2, Zbigniew Kołaciński2, Karolina Przybyłowska-Sygut1

This work was supported by grant NCBR no. PBS2/A5/31/2013

1 – Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Hallera 1 Square, 90-647 Lodz, Poland. 2 – Lodz University of Technology, Stefanowskiego Str. 18/22, Lodz 90-924, Poland.

Aim

Nanotechnology may develop a novel therapeutic strategies for selective cancer treatment. The main goal of the study was to develop the method for hyperthermic selective destruction of human colorectal cancer cells using ferromagnetic carbon nanotube (Fe-CNTs).

Method

The chemical vapour deposition (CVD) method was used to produce nanotubes followed by their biochemical functionalization. Human colorectal cancer cells HT-29 (ATTC) and normal colon mucosal epithelial cells NCM460 (InCell) were examined in vitro. In order to evaluate biological ability, the cytotoxicity of Fe-CNTs were examined using by resazurin toxicology assay (Sigma-Aldrich).

Results

Cells were treated with Fe-CNTs at the final concentration range of 0.05-5 mg/ml. We did not find cytotoxic effect on the viability of cells treated with ferromagnetic modified CNTs (P<0.05). It was also estimated no statistical differences in the sensitivity of colon cancer HT-29 cells in comparison to control cells after using Fe-CNTs. Future, Fe-CTNs with folic acid (FA) addressed to colon cancers expressing FA receptors will be used.

Conclusion

Therefore, we conclude that Fe-CNTs may be used as non-toxic ferromagnetic molecules with their practical therapeutic applications. The final result of our study would be the demonstration of the device and methodology for the Fe-CNTs hyperthermic treatment of colon cancer.

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