SO22: Colon polyps prevention and associated gut microbiota changes in a colorectal cancer animal model fed with functional meat foods containing prebiotics

Javier Fernández1, Estefanía Ledesma2, Enric Milán3, Pedro Costa3, Joaquín Monte2, Pablo Martínez-Camblor4, María Varela4, Adolfo Suárez4, Claudio J. Villar1, Felipe Lombó1.

1 – Research Group Biotechnology and Experimental Therapy based on Nutraceuticals. Department of Microbiology. University of Oviedo, Spain. 2 – El Hórreo Healthy Food SL. Noreña, Asturias, Spain. 3 – Cosfer SA. Barcelona, Spain. 4 – Digestive Tract Clinical Unit. Hospital Universitario Central de Asturias. Oviedo, Spain. 5 – Deparment of Statistics. University of Oviedo. Oviedo, Spain.

Aim

Diet is an important environmental factor for development of colorectal cancer. We try to demonstrate if daily consumption of the prebiotic fiber inulin is able to reduce occurrence of colorectal polyps in a chemical induced animal model for colon cancer (azoxymethane 10 mg/kg combined with DSS 3%).

Method

Two different functional meat matrixes have been developed: cooked ham (HAM, containing 10% inulin) and traditional chorizo sausage (TCS, containing 15.8% inulin).  These functional meat foods were given to experimental animal cohorts, together with conventional rat feed.  An absolute control diet (only rat feed) and two experimental control diets (feed plus normal meat matrixes) were also included.

Results

After the 17 weeks study, animals were sacrificed and colons examined. Functional meat products cohorts showed increased levels of intestinal short chain fatty acids, 48.94% less tumours and 57.45% less tumour mucosal area than control groups. Microbiome analyses also showed a clear modification in the case of functional diet cohorts, with a clear increase of diverse bacterial groups belonging to Bacteroidetes and Proteobacteria phyla.

Conclusion

The prebiotic fiber inulin is able to drastically reduce the appearance and extension of colon polyps in this animal model, producing big changes in the colon microbiome composition.

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