N79: Immune Microenvironment Of Colorectal Carcinoma

P. Janega1, 2, E. Gaal2, K. Giertlova2, J.Sedlak3, P. Babal1, 2

1 – Medirex Group Academy, n.p.o., Trnava, Slovak Republic. 2 – Department of Pathology, Faculty of Medicine Comenius University in Bratislava, Slovakia. 3 – Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.

 

Aim: The immune system plays crucial role in the development of the neoplastic diseases. Colorectal carcinoma is one of the most frequent oncological diseases with high mortality rate also in Slovak republic. Its development is the result of environmental, genetic and epigenetic changes accumulation leading to neoplastic transformation. Tumor-specific mutations manifest by neoantigens activating the immune system. The aim of the work was to evaluate the antitumor immune microenvironment in association of tumor grading.

Method: Archival surgical specimens of CRC were evaluated and graded according to the WHO criteria. Immunohistochemically detected CD4, CD8 and CD68 positive cells were evaluated morphometrically and expressed as % of the evaluated area.

Results: Neoplastic as well as the surrounding tissues were infiltrated by the three cell types in unchanged ratios, with predomonation of CD68+ histiocytes. With the increasing grade there was significant decrease of CD4+ and CD68+ cells and a clear decrease of CD8+ cells at the edge of significance, of infiltration of the tumor tissue. Changes in the peritumoral tissue infiltration were not significant.

Conclusion: Our findings support the idea of tumor suppressing activity of the anti tumor immunological response and that it plays an important role in progression of the neoplasm.

Supported by the APVV-14-0318 grant.

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